Optimization of electroporation-mediated transformation: Staphylococcus carnosus as model organism
نویسندگان
چکیده
منابع مشابه
Highly Efficient Electroporation-mediated Transformation into Edible Mushroom Flammulina velutipes
In this study, we developed an efficient electroporation-mediated transformation system featuring Flammulina velutipes. The flammutoxin (ftx) gene of F. velutipes was isolated by reverse transcription-PCR. pFTXHg plasmid was constructed using the partial ftx gene (410 bp) along with the hygromycin B phosphotransferase gene (hygB) downstream of the glyceraldehydes-3-phosphate dehydrogenase (gpd)...
متن کاملChlamydomonas as a Model Organism.
The unicellular green alga Chlamydomonas offers a simple life cycle, easy isolation of mutants, and a growing array of tools and techniques for molecular genetic studies. Among the principal areas of current investigation using this model system are flagellar structure and function, genetics of basal bodies (centrioles), chloroplast biogenesis, photosynthesis, light perception, cell-cell recogn...
متن کاملComplete Genome Sequence of Staphylococcus carnosus LTH 3730
Specific strains of the apathogenic coagulase-negative species Staphylococcus carnosus are frequently used as meat starter cultures, as they contribute to color formation and the production of aroma compounds. Here, we report the complete genome sequence of S. carnosus LTH 3730, a strain isolated from a fermented fish product.
متن کاملAgrobacterium-mediated and electroporation-mediated transformation of Chlamydomonas reinhardtii: a comparative study
BACKGROUND Chlamydomonas reinhardtii is an unicellular green alga used for functional genomics studies and heterologous protein expression. A major hindrance in these studies is the low level and instability of expression of nuclear transgenes, due to their rearrangement and/or silencing over time. RESULTS We constructed dedicated vectors for Agrobacterium-mediated transformation carrying, wi...
متن کاملOptimization of Agrobacterium-Mediated Transformation in Soybean
High transformation efficiency is a prerequisite for study of gene function and molecular breeding. Agrobacterium tumefaciens-mediated transformation is a preferred method in many plants. However, the transformation efficiency in soybean is still low. The objective of this study is to optimize Agrobacterium-mediated transformation in soybean by improving the infection efficiency of Agrobacteriu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2007
ISSN: 1364-5072,1365-2672
DOI: 10.1111/j.1365-2672.2006.03127.x